Characterization and Solubilization of Kaurenoic Acid Hydroxylase from Gibberella fujikuroi
نویسندگان
چکیده
منابع مشابه
Microbial transformation of ent-kaurenoic acid and its 15-hydroxy derivatives by the SG138 mutant of Gibberella fujikuroi.
Feeding experiments with ent-kaurenoic acid (4), 15 alpha-hydroxy-ent-kaurenoic acid (5), 15 beta-hydroxy-ent-kaurenoic acid (6), and mixtures of 4 plus 5 and 4 plus 6 were conducted using the SG138 mutant of Gibberella fujikuroi, to gain information about the phenotype of this unique strain. The biotransformation of 5 gave 7 beta,15 alpha-dihydroxykaurenolide (9) and 7 beta,15 alpha-dihydroxy-...
متن کاملRegulation of Gibberellin Biosynthesis in Gibberella fujikuroi.
Gibberellin production by Gibberella fujikuroi started only after the nitrogen source was depleted and ceased upon its renewal. Nitrogen repression of gibberellin biosynthesis is not an indirect effect of the growth arrest that follows the depletion of an essential nutrient because gibberellins were not produced upon depletion of phosphate. Mycelia produced gibberellins when suspended in a gluc...
متن کاملGlutamine Involvement in Nitrogen Control of Gibberellic Acid Production in Gibberella fujikuroi.
When the fungus Gibberella fujikuroi ATCC 12616 was grown in fermentor cultures, both intracellular kaurene biosynthetic activities and extracellular GA(3) accumulation reached high levels when exogenous nitrogen was depleted in the culture. Similar patterns were exhibited by several nonrelated enzymatic activities, such as formamidase and urease, suggesting that all are subject to nitrogen reg...
متن کاملProduction of Fumonisin B(inf1) and Moniliformin by Gibberella fujikuroi from Rice from Various Geographic Areas.
Gibberella fujikuroi strains isolated from rice in the United States, Asia, and other geographic areas were tested for sexual fertility with members of mating population D and for production of fumonisin B(inf1) and moniliformin in culture. Of the 59 field strains tested, 32 (54%) were able to cross with tester strains of mating population D, but only a few ascospores were produced in most of t...
متن کاملMorphological mutants of Gibberella fujikuroi for enhanced production of gibberellic acid.
AIMS To examine the production of gibberellic acid by selected morphological mutants of Gibberella fujikuroi in liquid cultures. METHODS AND RESULTS Mutants of G. fujikuroi having different morphological characteristics were selected after UV irradiation. The production of gibberellic acid by mutants that had different hyphal lengths was examined in shake flasks in media with different concen...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1993
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.101.3.925